Human activities are altering biodiversity and the nitrogen (N) cycle, affecting terrestrial carbon (C) cycling globally. Only a few specialized bacteria carry out nitrification�the transformation of ammonium (NH4+) to nitrate (NO3-), in terrestrial ecosystems, which determines the form and mobility of inorganic N in soils. However, the control of nitrification on C cycling in natural ecosystems is poorly understood. In an ecosystem experiment in the Patagonian steppe, we inhibited autotrophic nitrification and measured its effects on C and N cycling. Decreased net nitrification increased total mineral N and NH4+ and reduced NO3- in the soil. Plant cover (P